NP:P and NP:V Interactions of the Paramyxovirus Simian Virus 5 Examined Using a Novel Protein:Protein Capture Assay

AbstractUsing recombinant proteins extracted from mammalian cells, in a novel protein:protein binding assay, direct interaction of the nucleoprotein (NP) of simian virus 5 with the phosphoprotein (P) and V protein (V) was demonstrated. The amount of NP bound by V was found to be significantly less than that bound by P. Furthermore, preabsorption of NP with P removed the fraction of NP that could be bound by V, but preabsorption of NP with V did not remove all the NP that could be bound by P. These results suggested that V bound a subpopulation of the NP recognised by P. Further analysis revealed that P bound both soluble and homopolymeric forms of NP, while V bound only the soluble form; thus demonstrating that the binding sites on P and V, for soluble NP, are located within the N-terminal domain common to both P and V proteins. A monoclonal antibody, which recognised an epitope in the unique C-terminus of P, blocked the binding of P to polymeric NP but not to soluble NP. These results also suggest that there are two binding sites on NP for P; the site that interacts with the P/V common domain being either hidden or conformationally altered in polymeric NP

Models of Social Groups in Blogosphere Based on Information about Comment Addressees and Sentiments

This work concerns the analysis of number, sizes and other characteristics of groups identified in the blogosphere using a set of models identifying social relations. These models differ regarding identification of social relations, influenced by methods of classifying the addressee of the comments (they are either the post author or the author of a comment on which this comment is directly addressing) and by a sentiment calculated for comments considering the statistics of words present and connotation. The state of a selected blog portal was analyzed in sequential, partly overlapping time intervals. Groups in each interval were identified using a version of the CPM algorithm, on the basis of them, stable groups, existing for at least a minimal assumed duration of time, were identified.Comment: Gliwa B., Ko\'zlak J., Zygmunt A., Models of Social Groups in Blogosphere Based on Information about Comment Addressees and Sentiments, in the K. Aberer et al. (Eds.): SocInfo 2012, LNCS 7710, pp. 475-488, Best Paper Awar

Application of Sentinel-2A data for pasture biomass monitoring using a physically based radiative transfer model

A large proportion of the global land surface is covered by pasture. The advent of the Sentinel satellites program provides free datasets with good spatiotemporal resolution that can be a valuable source of information for monitoring pasture resources. We combined optical remote sensing data (proximal hyperspectral and Sentinel 2A) with a radiative transfer model (PROSAIL) to estimate leaf area index (LAI), and biomass, in a dairy farming context. Three sites in Southern England were used: two pasture farms that differed in pasture type and management, and a set of small agronomy trial plots with different mixtures of grasses, legumes and herbs, as well as pure perennial ryegrass. The proximal and satellite spectral data were used to retrieve LAI via PROSAIL model inversion, which were compared against field observations of LAI. The potential of bands of Sentinel 2A that corresponded with a 10 m resolution was studied by convolving narrow spectral bands (from a handheld hyperspectral sensor) into Sentinel 2A bands (10 m). Retrieved LAI, using these spectrally resampled S2A data, compared well with measured LAI, for all sites, even for those with mixed species cover (although retrieved LAI was somewhat overestimated for pasture mixtures with high LAI). This proved the suitability of 10 m Sentinel 2A spectral bands for capturing LAI dynamics for different types of pastures. We also found that inclusion of 20 m bands in the inversion scheme did not lead to any further improvement in retrieved LAI. Sentinel 2A image based retrieval yielded good agreement with LAI measurements obtained for a typical perennial ryegrass based pasture farm. LAI retrieved in this way was used to create biomass maps (that correspond to indirect biomass measurements by Rising Plate Meter (RPM)), for mixed-species paddocks for a farm for which limited field data were available. These maps compared moderately well with farmer-collected RPM measurements for this farm. We propose that estimates of paddock-averaged and within-paddock variability of biomass are more reliably obtained from a combined Sentinel 2A-PROSAIL approach, rather than by manual RPM measurements. The physically based radiative transfer model inversion approach outperformed the Normalised Difference Vegetation Index based retrieval method, and does not require site specific calibrations of the inversion scheme

Bias, accuracy, and impact of indirect genetic effects in infectious diseases

Selection for improved host response to infectious disease offers a desirable alternative to chemical treatment but has proven difficult in practice, due to low heritability estimates of disease traits. Disease data from field studies is often binary, indicating whether an individual has become infected or not following exposure to an infectious disease. Numerous studies have shown that from this data one can infer genetic variation in individuals’ underlying susceptibility. In a previous study, we showed that with an indirect genetic effect (IGE) model it is possible to capture some genetic variation in infectivity, if present, as well as in susceptibility. Infectivity is the propensity of transmitting infection upon contact with a susceptible individual. It is an important factor determining the severity of an epidemic. However, there are severe shortcomings with the Standard IGE models as they do not accommodate the dynamic nature of disease data. Here we adjust the Standard IGE model to (1) make expression of infectivity dependent on the individuals’ disease status (Case Model) and (2) to include timing of infection (Case-ordered Model). The models are evaluated by comparing impact of selection, bias, and accuracy of each model using simulated binary disease data. These were generated for populations with known variation in susceptibility and infectivity thus allowing comparisons between estimated and true breeding values. Overall the Case Model provided better estimates for host genetic susceptibility and infectivity compared to the Standard Model in terms of bias, impact, and accuracy. Furthermore, these estimates were strongly influenced by epidemiological characteristics. However, surprisingly, the Case-Ordered model performed considerably worse than the Standard and the Case Models, pointing toward limitations in incorporating disease dynamics into conventional variance component estimation methodology and software used in animal breeding

In search of late-stage planetary building blocks

Genetic contributions to the final stages of planetary growth, including materials associated with the giant Moon forming impact, late accretion, and late heavy bombardment are examined using siderophile elements. Isotopic similarities between the Earth and Moon for both lithophile and siderophile elements collectively lead to the suggestion that the genetics of the building blocks for Earth, and the impactor involved in the Moon-forming event were broadly similar, and shared some strong genetic affinities with enstatite chondrites. The bulk genetic fingerprint of materials subsequently added to Earth by late accretion, defined as the addition of ~0.5 wt.% of Earth's mass to the mantle, following cessation of core formation, was characterized by 187Os/188Os and Pd/Ir ratios that were also similar to those in some enstatite chondrites. However, the integrated fingerprint of late accreted matter differs from enstatite chondrites in terms of the relative abundances of certain other HSE, most notably Ru/Ir. The final ≤0.05 wt.% addition of material to the Earth and Moon, believed by some to be part of a late heavy bombardment, included a component with much more fractionated relative HSE abundances than evidenced in the average late accretionary component. Heterogeneous 182W/184Wisotopic compositions of some ancient terrestrial rocks suggest that some very early formed mantle domains remained chemically distinct for long periods of time following primary planetary accretion. This evidence for sluggish mixing of the early mantle suggests that if late accretionary contributions to the mantle were genetically diverse, it may be possible to isotopically identify the disparate primordial components in the terrestrial rock record using the siderophile element tracers Ru and Mo.NASA grants NNX13AF83G and NNA14AB07A NSF-CSEDI grants EAR1160728 and EAR1265169

Proprioceptor Pathway Development Is Dependent on MATH1

AbstractThe proprioceptive system provides continuous positional information on the limbs and body to the thalamus, cortex, pontine nucleus, and cerebellum. We showed previously that the basic helix-loop-helix transcription factor Math1 is essential for the development of certain components of the proprioceptive pathway, including inner-ear hair cells, cerebellar granule neurons, and the pontine nuclei. Here, we demonstrate that Math1 null embryos lack the D1 interneurons and that these interneurons give rise to a subset of proprioceptor interneurons and the spinocerebellar and cuneocerebellar tracts. We also identify three downstream genes of Math1 (Lh2A, Lh2B, and Barhl1) and establish that Math1 governs the development of multiple components of the proprioceptive pathway

Island resource exploitation by the ancient Maya during periods of climate stress, Ambergris Caye, Belize

Ancient Maya societies experienced a period of reorganisation and change in settlement patterns associated with social and climate instability at the end of the Classic period (750-1000 CE) and the subsequent Postclassic period (1200-1500 CE). Although it has been proposed that severe droughts and the breakdown of Classic political systems caused a migration of populations towards the coast, we have little evidence of the nature of land-use at coastal sites. Our understanding of subsistence on islands has been shaped by archaeological research indicating marine-based diets, with maize imported from the mainland. Here we provide, for the first time, palaeoecological proxy data that inform on ancient Maya land-use on an island site, located on Ambergris Caye, Belize. Using pollen and charcoal proxies, we present over 6000 years of environmental change and land-use history. Our reconstruction reveals evidence of cultivation, beginning at 2900 BCE and culminating during the Postclassic Period. We demonstrate that periods of higher land-use intensity correlate with climate instability, which corroborates archaeological evidence of migration to coastal locations. We hypothesize that the diverse marine and terrestrial environments of the island provided sustainable resources for the mainland Maya to use during times of both political and climatic stress

Changes in the trajectory of Long Covid symptoms following COVID-19 vaccination: community-based cohort study

OBJECTIVE: To estimate associations between COVID-19 vaccination and Long Covid symptoms in adults who were infected with SARS-CoV-2 prior to vaccination. DESIGN: Observational cohort study using individual-level interrupted time series analysis. SETTING: Random sample from the community population of the UK. PARTICIPANTS: 28,356 COVID-19 Infection Survey participants (mean age 46 years, 56% female, 89% white) aged 18 to 69 years who received at least their first vaccination after test-confirmed infection. MAIN OUTCOME MEASURES: Presence of Long Covid symptoms at least 12 weeks after infection over the follow-up period 3 February to 5 September 2021. RESULTS: Median follow-up was 141 days from first vaccination (among all participants) and 67 days from second vaccination (84% of participants). First vaccination was associated with an initial 12.8% decrease (95% confidence interval: -18.6% to -6.6%, p<0.001) in the odds of Long Covid, with the data being compatible with both increases and decreases in the trajectory (+0.3% per week, 95% CI: -0.6% to +1.2% per week, p=0.51) after this. Second vaccination was associated with an 8.8% decrease (95% CI: -14.1% to -3.1%, p=0.003) in the odds of Long Covid, with the odds subsequently decreasing by 0.8% (-1.2% to -0.4%, p<0.001) per week. There was no statistical evidence of heterogeneity in associations between vaccination and Long Covid by socio-demographic characteristics, health status, whether hospitalised with acute COVID-19, vaccine type (adenovirus vector or mRNA), or duration from infection to vaccination. CONCLUSIONS: : The likelihood of Long Covid symptoms reduced after COVID-19 vaccination, and there was evidence of a sustained improvement after the second dose, at least over the median follow-up time of 67 days. Vaccination may contribute to a reduction in the population health burden of Long Covid, though longer follow-up time is needed

The Relationship between the Extent of Collaboration of General Practitioners and Pharmacists and the Implementation of Recommendations Arising from Medication Review A Systematic Review

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